副教授
    杨  坤 副教授 - 简介

    副教授

    制药与生物工程系

    四川大学化学工程学院

    四川省成都市武侯区一环路南一段24号,610065

    Email: cookyoung@scu.edu.cn

    电话: +86 180-8107-8027

    个人简述:具有超过十五年的生物工程相关领域的研究经验;研究兴趣广泛,涉及生物分离、功能磁性微粒的制备及应用、环境微生物及微生物生态等多个领域;在Journal Chromatography ABiochemical Engineering JournalSeparation and Purification TechnologyChemical Engineering ScienceIndustrial & Engineering Chemistry ResearchJournal of BiotechnologyAnalytical Biochemistry Applied and Environmental MicrobiologyJournal of Applied MicrobiologyFrontiers in Microbiology等国际知名的学术期刊上发表SCI论文近20篇,他引总次数超过250次。

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杨  坤 副教授 -- 个人学历、简历

学历

博士(硕博连读) 生物化工,天津大学,2007

导师:孙彦 教授(长江学者)

博士论文题目:《蛋白质离子交换色谱的吸附传质动力学研究》

学士 生物化工,天津大学,2001

工作经历

201311月至今 副教授,四川大学化学工程学院,制药与生物工程系

20117-20135 博士后,夏威夷大学马诺亚分校,市政与环境工程系(Department of Civil and Environmental Engineering, University of Hawaii at Manoa

20085-20116 博士后,夏威夷大学马诺亚分校,分子生物科学与生物工程系(Department of Molecular Biosciences & Bioengineering, University of Hawai'i at Manoa

20079-20084 助理教授,厦门大学化学化工学院,化学工程与生物工程系

杨  坤 副教授 -- 科学研究

研究方向/兴趣

1. 生物分离:色谱分离过程的动力学研究;

2. 功能磁性微粒及相关应用:磁分离、多酶固定化、医学诊断、磁性免疫分析等;

3. 环境微生物及微生物生态:环境致病菌的高灵敏度检测、污水抗生素抗药组、抗生素土壤环境效应等。

科研项目

参研及主研项目

20049-20075

· 共聚焦激光扫描显微镜辅助多组分蛋白质吸附和色谱机理研究,国家自然科学基金,项目编号:20306024 2004-2006

· 蛋白质静电吸附的多尺度研究,高等学校博士学科点专项科研基金,项目编号: 200500560012005-2007

· 蛋白质离子交换吸附平衡的多尺度方法学研究,国家自然科学基金,项目编号:204760822005-2007

20085-20116

· 环境中Ralstonia solanacearum菌株集成化快速检出系统的开发,美国农业部NRI支助;(Development of an integrated rapid detection system for discrimination of Ralstonia solanacearum strains in the environment. No: 2005-55605-16683, USDA NRI grant, 2006-2011.

· 一种高效生产植物源抗体的集成化方法,美国农业部TSTAR研究项目;(An Integrated Approach for Efficient Production of Plant-derived Antibodies. No: 2008-34135-19407, USDA TSTAR research program, 2008-2011.

· 金属螯合磁性纳米颗粒的制备及应用;

· 自组装的功能磁性纳米油体。

20117月至今

· 市政污水中大肠杆菌致病基因的丰度及多态性;(Abundance and diversity for E. coli virulence genes in municipal wastewater.)

· 人为因素暴露下土壤抗生素抗性的分子演化,美(国)-以(色列)两国科学基金会(项目);(Molecular evolution of antibiotic resistance in soil exposed to anthropogenic factors. United States-Israel Binational Science Foundation

· 夏威夷海滩沙中粪便指示菌及水生致病菌的存活,美国夏威夷卫生署(项目);(Survival of Fecal Indicator Bacteria and Waterborne Pathogens in Hawaiian Beach Sand. Hawaii Department of Health

主持完成项目

· 市政污水抗生素抗药组研究,YJ201355, 四川大学引进人才科研启动经费资助项目,2014-2016

主持在研项目

· 鸟类迁徙介导的环境抗生素抗性散播及内在机制研究,21677104,国家自然科学基金面上项目,2017-2020

受邀审稿杂志

· Enzyme and Microbial TechnologyJournal of Molecular Catalysis B

杨  坤 副教授 -- 发表论文

期刊论文

1. Yang K, Shi Q and Sun Y (2006) Modeling and simulation of protein uptake in cation exchanger visualized by confocal laser scanning microscopy. Journal Chromatography A. 1136, 19-28

2. Yang K and Sun Y (2007) Structured parallel diffusion model for intraparticle mass transport of proteins to porous adsorbent. Biochemical Engineering Journal. 37, 298-310

3. Lu M, Bai S, Yang K and Sun Y (2007) Synthesis and characterization of magnetic polymer microspheres with a core–shell structure. China Particuology. 5, 180-185

4. Yang K and Sun Y (2008) Optics-intrinsic double-circle phenomenon in protein adsorption visualized by confocal laser scanning microscopy. Biochemical Engineering Journal. 39, 258–266

5. Sun Y and Yang K (2008) Analysis of mass transport models based on the Maxwell-Stefan theory and the Fick’s law for protein uptake to porous anion exchanger. Separation and Purification Technology. 60, 180–189

6. Yang K, Bai S and Sun Y (2008) Protein adsorption dynamics in cation-exchange chromatography quantitatively studied by confocal laser scanning microscopy. Chemical Engineering Science. 63, 4045 – 4054

7. Huang JL, Lin LQ, Li QB, Sun DH, Wang YP, Lu YH, He N, Yang K, Yang X, Wang HX, Wang WT and Lin WS (2008) Continuous-flow biosynthesis of silver nanoparticles by lixivium of sundried Cinnamomum camphora leaf in tubular microreactors. Industrial & Engineering Chemistry Research. 47, 6081-6090

8. Fan HY, Yang K, Zhang CX, Wang X, He N, Wang YP, Sun DH and Li QB (2008) Separation and purification of catalpol from Radix Rehmanniae. Journal of Chemical Industry and Engineering (China). 59(9), 2283-2288 (in Chinese with English abstract)

9. Wang X, Yang K, Wang ZS, He N, Wang YP, Sun DH and Li QB (2009) Separation and purification of flavonoids from Cinnamomum Camphora leaves via microwave-assisted extraction and macroporous resin adsorption. Transactions of the CSAE. 25(Supp. 1): 138-141 (in Chinese with English abstract)

10. Wang HT, Li QB, He N, Wang YP, Sun DH, Shao WY, Yang K and Lu YH (2009) Removal of anthraquinone reactive dye from wastewater by batch hydrolytic-aerobic recycling process. Separation and Purification Technology. 67, 180-186

11. Yang K, Xu NS and Su WW (2010) Co-immobilized enzymes in magnetic chitosan beads for improved hydrolysis of macromolecular substrates under a time-varying magnetic field. Journal of Biotechnology. 148, 119-127

12. Yang K and Su WW (2011) Facile synthesis of metal-chelating magnetic nanoparticles by exploiting organophosphorus coupling. Analytical Biochemistry. 408, 175-177

13. Yang K, Jenkins DM and Su WW (2011) Rapid concentration of bacteria using submicron magnetic anion exchangers for improving PCR-based multiplex pathogen detection. Journal of Microbiological Methods. 86, 69-77

14. Cui H*, Yang K*, Pagaling E. and Yan T. (2013). Spatial and Temporal Variations of Enterococci Abundance and Its Relationship with Microbial Community in Hawai’i Beach Sand and Water. Applied and Environmental Microbiology. 79, 3601-3609 (*equal contributors)

15. Yang K, Pagaling E and Yan T (2014) Estimating the Prevalence of Potential Enteropathogenic Escherichia coli and Intimin Gene Diversity in Bacterial Communities by Monitoring Sanitary Sewage. Applied and Environmental Microbiology, 80, 119-127

16. Pagaling E, Yang K and Yan T (2014) Pyrosequencing reveals correlations between extremelyacidophilic bacterial communities with hydrogen sulfide concentrations, pH and inert polymer coatings at concrete sewer crown surfaces. Journal of Applied Microbiology, 117, 50-64

17. Xu WD, Zhang WJ, Han D, Cui HL* and Yang K* (2015) Halorussus rubersp. nov., isolated from an inland salt lake of China. Arch Microbiol, 197: 91-95 *corresponding authors

18. Gatica J**, Yang K**, Pagaling E, Jurkevitch E, Yan T and Cytryn E* (2015) Resistance of Undisturbed Soil Microbiomes to Ceftriaxone Indicates Extended Spectrum β-Lactamase Activity. Front. Microbiol. 6:1233. doi: 10.3389/fmicb.2015.01233 (**equal contributors)

19. Yang K* (2016) Dynamic binary protein adsorption in ion-exchange media depicted with a parallel diffusion model derived from Maxwell–Stefan theory. Chemical Engineering Science 139: 163–172

20. Pagaling E, Gatica J, Yang K, Cytryn E, Yan T* (2016) Phylogenetic diversity of ceftriaxone resistance and the presence of extended-spectrum b-lactamase genes in the culturable soil resistome. Journal of Global Antimicrobial Resistance 6:128–135

会议论文/海报

1. Yang K, Yang Z, Shi QH and Sun Y; Visualizing distribution of protein in chromatographic adsorbent beads using confocal laser scanning microscopy. Proceedings of the 1st National Annual Conference on Chemical Engineering and Biochemical Engineering, Nanjing, 2004; C066. (Oral presentation)

2. Yang K, Yang Z, Shi QH and Sun Y; Protein diffusion behavior in porous medium visualized by confocal laser scanning microscopy. Nationwide Doctoral Forum of Chemical Engineering in China, Tianjin University, 2005; 190. (Oral presentation)

3. Yang K, Pagaling E, Li Y and Yan T; Abundance and diversity of Escherichia coli intimin gene (eaeA) in Honolulu municipal wastewater. Hawaii Water Environment Association (HWEA) Annual Conference, 2013. (Poster presentation)

4. Yang K, Cui H, Pagaling E, and Yan T; Spatial and temporal variations of enterococci abundance and its relationship with microbial community in Hawai’i beach sand and water. Hawaii Water Environment Association (HWEA) Annual Conference, 2013. (Poster presentation)

5. Pagaling E, Yang K, and Yan T; Microbes involved in concrete sewer pipe corrosion. Hawaii Water Environment Association (HWEA) Annual Conference, 2013. (Poster presentation)

杨  坤 副教授 -- 教学方面工作

课堂教学

1. 数学物理方程(研究生课程),厦门大学,2008

2. 环境生物技术(本科生课程),四川大学,2014年春起

3. 代谢工程(本科生课程),四川大学,2014年春起

4. 生物信息学(本科生课程),四川大学,2015年春起

5. 制药工程设计(本科生课程),四川大学,2015年秋起

学生指导

硕士研究生

饶大伟, 20149月至今;

伍娇, 20159月至今;

本科生毕业论文(设计)

2014

陈鹏, 2010级生物工程,20142-6月,《成都市锦江水样中粪便指示菌的分离、鉴定及保藏》;

王爽, 2010级制药工程,20142-6月,《锦江(成都)水体粪便指示菌抗生素抗药性研究》;

2015

黄烨, 2011级生物工程,20152-6月,《抗生素抗性通过野生鹭鸟活动从污染水体向周边环境扩散的迹象及其内在机制》(校优论文)

李玉贤, 2011级制药工程,20152-6月,《EVE法生产磺胺嘧啶的工艺优化设计》;

拉西, 2011级生物工程,20152-6月,《一种用于血浆游离DNA提取的磁性纳米颗粒的制备及表征》;

王起正, 2011级生物工程,20152-6月,《抗生素土壤可降解性研究初步》。

2016

王倍, 2012级制药工程,20162-6月,《胰酶原料药的车间设计》(优秀毕设)

黄慧, 2012级生物工程,20162-6月,《基于磁性纳米颗粒的微生物安全性检测方法》

朱云帆, 2012级制药工程,20162-6月, 《环境样品中抗生素抗性基因的q-PCR定量》

地址:四川省成都市一环路南一段24号 邮编:610065 电话:028-85405222 网站浏览